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KMID : 1161519980020030367
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Animal Cells and Systems
1998 Volume.2 No. 3 p.367 ~ p.370
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Purification and characterization of apolipophorin-III in the hemolymph of the wax moth, galleria Mellonella L.
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Lee Su-Jin
Lee In-Hee
Kang Chang-Soo
Choi Chung-Sik
Yun Hwa-Kyung
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Abstract
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Two molecular species of apolipophorin?III (apoLp?III) were purified from the last instar larval hemolymph of Galleria mellonella by gel permeation chromatography (Sephadex G?100), ion exchange chromatography (DE?52), heat treatment (901 for 30min) and Mono S FPLC, and were named apoLp?III?a and apoLp?III?b, respectively. They were indistinguishable by SDS?PAGE but could be separated by native PAGE. The molecular mass of apoLp?III determined by SDS?PAGE was approximately 18kDa. The N?terminal amino acid sequence of apoLp?lll?b revealed high similarities with the apoLp?III from Manduca sexta.
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KEYWORD
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Galleria mellonella, Hemolymph, Apolipophorin-lll
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